ERA Testing for repeated failed pregnancies

RIF (recurrent or repeated implantation failure) may be devastating. Some patients have three or more rounds of IVF, and everything appears to go well: a good number of eggs at retrieval, a good fertilisation rate, the embryos appear to be healthy and of high quality, the uterine lining appears to be healthy and high quality, the transfer goes smoothly, but the pregnancy never “sticks.”

ERA Testing for repeated failed pregnancies

It may also be a difficult and unpleasant condition for reproductive specialists who are attempting to identify a reason. Patients seek answers, and physicians want to assist them. Fortunately, new tests and technologies are continuously being developed to assist reproductive professionals in providing some of these answers and assisting patients in finding the correct road to conception. One of these new advancements is the ERA test, which provides patients dealing with implantation failure with fresh knowledge and hope. For the best IVF center in Delhi book your appointment at World ivf center.



What factors contribute to implantation failure?

There are various circumstances that might lead to implantation failure. Most fall into one of two types.

Genetic abnormalities: The most prevalent problem is a genetic or chromosomal defect with the embryo, especially in older women who use their own eggs. The risk of chromosomal abnormalities in ageing eggs is substantially greater. This explains why implantation rates decrease with patient age: the typical implantation rate in patients under 35 using their own eggs is about 50%. By the time women are 41-42 years old, that percentage has dropped to roughly 12%. There may be a genetic abnormality with the sperm in rare cases. If a patient has RIF, genetic testing, such as Pre-Implantation Genetic Screening (PGS), is typically the first line of treatment. Embryologists may examine the embryos for chromosomal problems before implanting them, which can significantly boost the implantation rate. Donor eggs are another incredibly successful alternative in this sort of circumstance. Call and book your appointment with the IVF centre in south Delhi at World IVF centre.

Uterine receptivity difficulties: If there does not seem to be a genetic cause for implantation failure, testing will normally move on to uterine receptivity concerns. These may be caused by a variety of factors, including physical difficulties with the form and/or location of the uterus, hormone abnormalities or imbalances, thyroid or immunological functions, or problems with the endometrial lining itself. There are several tests that may assist physicians in narrowing down the problem. One of the most recent is the ERA test.

What exactly is the ERA test?

Endometrial Receptivity Analysis (ERA) is an abbreviation for Endometrial Receptivity Analysis (or Array). It is a genetic test that utilises a tiny sample of a woman’s endometrial tissue to determine if her endometrial lining is ready to receive an implanting embryo.

Each woman has a “implantation window” in her cycle that lasts a few days, usually between the 19th and 23rd day of her menstrual cycle. During the luteal or secretory phase, the ovaries produce progesterone. In order to establish an ideal environment for implantation, progesterone induces several subtle but significant modifications to the uterine lining. Proteins are produced, causing the lining to thicken and become more responsive. This narrow window occurs at the predicted period in around 84 percent of women. In the case of IVF cycles, this could mean that the embryo transfer is taking place on the wrong day, when the window hasn’t yet opened or has already closed, resulting in implantation failure.

What is the ERA test and how does it work?

When the endometrial lining is responsive, the endometrial cells’ genetic material has a distinct “expression,” which implies that they may produce more or less of specific kinds of RNA. The researchers examined the expression of 236 genes per sample and created a database of over 12,000 endometrial tissue samples to determine the quantities of RNA generated at various stages of the cycle. They then searched for patterns using advanced computer algorithms until they could reliably classify a sample as “Receptive” or “Non-Receptive” based on its specific expression profiles. This test is highly repeatable, which implies that the findings are the same whether samples are examined at the same point of the cycle but months apart. Doctors can use ERA to determine whether a woman’s “implantation window” is occurring as expected or whether the transfer date needs to be moved to ensure the best “sync” between the embryo and the uterine lining.

How is the ERA test carried out?

Endometrial Receptivity Analysis necessitates the collection of a sample of the uterine lining at a precise point in the cycle.

It is also possible to employ fertility drugs, such as supplementary oestrogen and progesterone, to prepare the uterine lining. The progesterone is begun on Day Zero, and the test is performed five days later.

Endometrial biopsy is the process used to get the tissue sample. A thin catheter is introduced through the cervix into the uterine cavity, and a plunger in the catheter creates suction to extract a microscopic sample of the endometrial lining into the catheter. Many women find this part of the procedure to be quite unpleasant, but it is a relatively quick procedure. Following that, the sample is delivered to the lab for examination utilising Next Generation Sequencing technology. The results will be available in around three weeks. If the tissue was found to be “Non-Receptive,” the test should be performed earlier or later until the ideal time is found.

After determining the patient’s receptive window, tailored embryo transfer (pET) timing may be employed.

The disappointment and distress produced by recurrent implantation failure is frequently intense after such a significant emotional, physical, and financial effort. If you or someone you care about has battled with inexplicable failed IVF cycles, talk about your fertility specialist about ERA. It provides you with a fresh data point, and knowledge is power. After repeated implantation failures, sometimes a one-day change is all that is required to achieve results.


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