Prerequisites for semen collection

A semen analysis evaluates ejaculated sperm, and 2-3 may be required to obtain a complete baseline because sperm counts might change from one day to the next. The process is an essential component of male fertility testing, and the results can identify whether or not male factor infertility exists. Call and book your appointment with IVF specialist In lajpat nagar at World IVF centre.

Prerequisites for semen collection

What exactly does a sperm analysis look for?

  • 1.5 ml to 5.0 ml in volume
  • Concentration (number of sperm per milliliter of ejaculate): more than 20 million/ml
  • Motility (percentage of sperm moving): 50% or higher
  • Forward progression (the strength of the migrating sperm’s forward movement): 2+ or more
  • Morphology (percentage of regularly shaped sperm): WHO guidelines require 30 percent or more normal forms, while Kruger rigorous requirements require 14 percent or more.
  • Minimal clumping
  • The presence of white or red blood cells is negligible.
  • There is no hyperviscosity (thickening of seminal fluid)
  • White blood cells at concentrations less than 1×106/ml

How can I get ready for a sperm analysis?

  • For at least 3-5 days, you will be asked to refrain from both intercourse and masturbation.
  • Your fertility doctor may also advise you to make changes to your lifestyle, such as avoiding alcohol, caffeine, and certain medications in the days preceding up to the sample collection.
  • Before collecting the sample, you must properly wash your hands.
  • The specimen should be placed in a sterile specimen pot (provided by your fertility center). The pot must be tagged with your full name, date of birth, and ejaculation date and time. The sample will not be analysed if this information is missing.
  • If you opt to ejaculate in the privacy and comfort of your own home, keep in mind that the sample must be kept warm and brought to your fertility centre within one hour following ejaculation. Furthermore, because condoms contain spermicide, they cannot be used to transport the sample.
  • You will set an appointment as part of the reproductive workup to either produce your sample in the office or drop it off at the clinic or lab after producing a sample at home. When it comes to sperm, timing is everything, so plan appropriately.
  • You must abstain from sexual activity for at least two days, but no longer than five to seven days, before your sample is collected. This means no intercourse or any form of ejaculation, including masturbation. Each physician may have different abstinence standards, so be sure to verify with your clinic. Abstinence for longer or shorter periods of time may result in a lower count, as well as decreased sperm motility or movement. When compared to samples produced after shorter or longer durations of abstinence, samples produced after two days of abstinence usually exhibit the highest number of motile sperm with the greatest forward velocity. Some men believe that preserving all of their sperm for the day of their test is preferable, but this is a significant mistake: older ones begin to die if ejaculations are uncommon, and the percentage of viable sperm falls with growing abstinence.
  • You’ll also have to give up some (perhaps unhealthy) behaviors. It is preferable to avoid smoking, drinking, and, of course, drugs for 10 days before your sperm collection (you may want to consider these lifestyle changes even further in advance).
  • Specific factors that may have an impact on the quality of your sperm sample include:
  • Cimetidine (Tagamet), male and female hormones (testosterone, oestrogen), sulfasalazine, nitrofurantoin, and various chemotherapy medications are examples of medications.
  • Caffeine, alcohol, cocaine, marijuana, and cigarettes are all stimulants. Some medicines, such as anabolic steroids, can have an impact on sperm production.
  • Masturbation is most likely the method you will use to acquire your sperm specimen. If you have severe male infertility with little or no sperm, a surgical procedure such as micro surgical epididymal sperm aspiration (MESA) or testicular sperm aspiration (TESA) may be necessary. Most doctors recommend that you ejaculate directly into the sample cup provided, rather than using a condom. If you must wear a condom, your laboratory will supply you with a special semen collecting condom (that does not contain spermicide).

There are a few guidelines to follow when obtaining your sperm sample:

  • First, take a shower to get a fresh start. Then, just before you get started, double-check that your hands and penis are clean. Wash them with soap and then thoroughly rinse them with water.
  • You must be a purist. You should not use any lubricant unless the clinic provides it. This includes saliva as well. Also, as previously said, do not collect your sperm in a condom (the spermicidal agents will alter the results of the analysis).
  • You must ejaculate into a sterile container given by your clinic or lab. Avoid touching the inside of the cup and attempt to get the first part of your ejaculation into the cup, which is regarded to be the most sperm-rich. If sperm spills, do not try to transfer it to your cup.
  • Put the lid on your jar as soon as you’ve gathered your sample. Ensure that your name, date of birth, time, and date of sample are all clearly printed on the cup. A sticker from your medical chart will be placed on the cup and lid by many clinics. Make certain that it is correct.


If you collect your sample outside of your clinic, you must provide it to the laboratory within one hour following ejaculation. Sperm does not have a long life outside of the body or in temperature-changing conditions. Delays in transporting sperm and exposure to different temperatures will result in a reduced overall motile sperm count and poor cryopreservation of the sperm.

Keep your sperm sample as close to body temperature as feasible. If the sperm sample becomes too cold or too hot, the sperm motility value will be incorrectly low.

Keep your specimen container upright in a plastic bag with the top well closed. The specimen should never be kept in a handbag, pocket, or briefcase.

Some clinics will need you to create the sperm sample on-site to ensure that you can produce a sample for a fertility cycle and that you get the greatest possible sample. Book your appointment for infertility treatment in Delhi at World IVF center.

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